InterPro : IPR011548

Name  3-hydroxyisobutyrate dehydrogenase Short Name  IsoBut3OH_DH
Type  Family Description  3-hydroxyisobutyrate dehydrogenase is an enzyme that catalyzes the NAD+-dependent oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde of the valine catabolism pathway. In Pseudomonas aeruginosa, 3-hydroxyisobutyrate dehydrogenase (mmsB) is co-induced with methylmalonate-semialdehyde dehydrogenase (mmsA) when grown on medium containing valine as the sole carbon source. The positive transcriptional regulator of this operon (mmsR) is located upstream of these genes and has been identified as a member of the XylS/AraC family of transcriptional regulators []. 3-hydroxyisobutyrate dehydrogenase shares high sequence homology to the characterised 3-hydroxyisobutyrate dehydrogenase from rat liver []with conservation of proposed NAD+ binding residues at the N terminus (G-8,10,13,24 and D-31). This enzyme belongs to the 3-hydroxyacid dehydrogenase family, sharing a common evolutionary origin and enzymatic mechanism with 6-phosphogluconate []. HIBADH exhibits sequence similarity to the NAD binding domain of 6-phosphogluconate dehydrogenase.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

3 Contains

Id Name Short Name Type
IPR006115 6-phosphogluconate dehydrogenase, NADP-binding 6PGDH_NADP-bd Domain
IPR013328 Dehydrogenase, multihelical DH_multihelical Domain
IPR002204 3-hydroxyisobutyrate dehydrogenase-related, conserved site 3-OH-isobutyrate_DH-rel_CS Conserved_site

0 Found In

1 Parent Features

Id Name Short Name Type
IPR015815 Hydroxy monocarboxylic acid anion dehydrogenase, HIBADH-type HIBADH-type Family

3 Publications

First Author Title Year Journal Volume Pages
Rougraff PM Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase. Evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases. 1989 J Biol Chem 264 5899-903
Steele MI Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase. 1992 J Biol Chem 267 13585-92
Hawes JW Structural and mechanistic similarities of 6-phosphogluconate and 3-hydroxyisobutyrate dehydrogenases reveal a new enzyme family, the 3-hydroxyacid dehydrogenases. 1996 FEBS Lett 389 263-7

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)