InterPro : IPR018222

Name  Nuclear transport factor 2, eukaryote Short Name  Nuclear_transport_factor_2_euk
Type  Domain Description  Ran () is an evolutionary conserved member of the Ras superfamily of small GTPases that regulates all receptor-mediated transport between thenucleus and the cytoplasm. Importreceptors bind their cargos in the cytoplasm where the concentration of RanGTP is low and release their cargos in thenucleus where the concentration of RanGTP is high []. Export receptors respond to Ran GTP in the oppositemanner. Nuclear transport factor 2 (NTF2) is a homodimer of approximately 14kDa subunits which stimulates efficient nuclear importof a cargo protein. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins. NTF2 binds to RanGDPsufficiently strongly for the complex to remain intact during transport through NPCs,but the interaction between NTF2 and FxFG nucleoporins is much more transient,which would enable NTF2 to move through the NPC by hopping from one repeat toanother [, ].NTF2 folds into a cone with a deep hydrophobic cavity, the opening of which is surrounded by several negatively charged residues. RanGDP binds to NTF2 by inserting a conserved phenylalanine residue into the hydrophobic pocket of NTF2 and making electrostatic interactions with the conserved negatively charged residues that surround the cavity.This entry contains predominantly eukaryotic proteins. The following proteins contain a region similar to NTF2:Eukaryotic NXF proteins []. These are nuclear mRNA export factors. These proteins contain, in addition to a NTF2 domain, a number of leucine-rich repeats and a UBA domain.Eukaryotic NXT1/NXT2 proteins []. These proteins are stimulators of protein export for NES-containing proteins. The also play a role in mRNA nuclear export. They heterodimerize with NFX proteins. In contrast to NTF2, NXT1 and NXT2 preferentially bind RanGTP.Eukaryotic Ras-GTPase-activating protein (GAP)-binding proteins (G3BP's). These proteins contain one NTF2 domain and one RRM (see ).

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

0 Found In

1 Parent Features

Id Name Short Name Type
IPR002075 Nuclear transport factor 2 NTF2 Domain

4 Publications

First Author Title Year Journal Volume Pages
Steggerda SM Regulation of nuclear import and export by the GTPase Ran. 2002 Int Rev Cytol 217 41-91
Stewart M Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2). 2000 Cell Struct Funct 25 217-25
Quimby BB The interaction between Ran and NTF2 is required for cell cycle progression. 2000 Mol Biol Cell 11 2617-29
Herold A TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture. 2000 Mol Cell Biol 20 8996-9008

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)