InterPro : IPR001154

Name  DNA topoisomerase II, eukaryotic-type Short Name  TopoII_euk
Type  Family Description  DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single- or double-strand breaks, crossing the strands through one another, then resealing the breaks []. These enzymes have several functions: to remove DNA supercoils during transcription and DNA replication; for strand breakage during recombination; for chromosome condensation; and to disentangle intertwined DNA during mitosis [, ]. DNA topoisomerases are divided into two classes: type I enzymes (; topoisomerases I, III and V) break single-strand DNA, and type II enzymes (; topoisomerases II, IV and VI) break double-strand DNA [].Type II topoisomerases are ATP-dependent enzymes, and can be subdivided according to their structure and reaction mechanisms: type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as for introducing both negative and positive supercoils [].This entry represents DNA topoisomerase II enzymes from eukaryotes and viruses. Topoisomerase II primarily functions in introducing negative supercoils into DNA, and is of particular importance during the segregation of chromosomes during mitosis [, ]. In eukaryotes and viruses, this enzyme occurs as a single polypeptide, with the N-terminal portion (homologous to subunit B of bacterial topoisomerase II, or gyraseB) responsible for ATPase activity and the C-terminal portion (homologous to subunit A of bacterial topoisomerase II, or gyraseA) responsible for the breaking-rejoining function through its capacity to form protein-DNA bridges. In mammals, there are at least two isozymes of this enzyme, topoisomerases II-alpha and II-beta, which are similar in structure and catalytic properties [, ]. The alpha isoform is involved in chromosome condensation and segregation.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



2 Child Features

Id Name Short Name Type
IPR028467 DNA topoisomerase II-beta Top2b Family
IPR028466 DNA topoisomerase II-alpha Top2a Family

5 Contains

Id Name Short Name Type
IPR002205 DNA topoisomerase, type IIA, subunit A/C-terminal Topo_IIA_A/C Domain
IPR013760 DNA topoisomerase, type IIA-like domain Topo_IIA_like_dom Domain
IPR013759 DNA topoisomerase, type IIA, central domain Topo_IIA_cen_dom Domain
IPR018522 DNA topoisomerase, type IIA, conserved site TopoIIA_CS Conserved_site
IPR013758 DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta Topo_IIA_A/C_ab Domain

0 Found In

1 Parent Features

Id Name Short Name Type
IPR001241 DNA topoisomerase, type IIA Topo_IIA Family

9 Publications

First Author Title Year Journal Volume Pages
Roca J The mechanisms of DNA topoisomerases. 1995 Trends Biochem Sci 20 156-60
Champoux JJ DNA topoisomerases: structure, function, and mechanism. 2001 Annu Rev Biochem 70 369-413
Gadelle D Phylogenomics of type II DNA topoisomerases. 2003 Bioessays 25 232-42
Wang JC Cellular roles of DNA topoisomerases: a molecular perspective. 2002 Nat Rev Mol Cell Biol 3 430-40
Watt PM Structure and function of type II DNA topoisomerases. 1994 Biochem J 303 ( Pt 3) 681-95
Berger JM Structure and mechanism of DNA topoisomerase II. 1996 Nature 379 225-32
Porter AC Topoisomerase II: untangling its contribution at the centromere. 2004 Chromosome Res 12 569-83
Austin CA Eukaryotic DNA topoisomerase II beta. 1998 Bioessays 20 215-26
Sakaguchi A Functional compatibility between isoform alpha and beta of type II DNA topoisomerase. 2004 J Cell Sci 117 1047-54

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)