InterPro : IPR018522

Name  DNA topoisomerase, type IIA, conserved site Short Name  TopoIIA_CS
Type  Conserved_site Description  DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single- or double-strand breaks, crossing the strands through one another, then resealing the breaks []. These enzymes have several functions: to remove DNA supercoils during transcription and DNA replication; for strand breakage during recombination; for chromosome condensation; and to disentangle intertwined DNA during mitosis [, ]. DNA topoisomerases are divided into two classes: type I enzymes (; topoisomerases I, III and V) break single-strand DNA, and type II enzymes (; topoisomerases II, IV and VI) break double-strand DNA [].Type II topoisomerases are ATP-dependent enzymes, and can be subdivided according to their structure and reaction mechanisms: type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as for introducing both negative and positive supercoils [].DNA topoisomerase II () [, , ]is one of the two types of enzyme that catalyze the interconversion of topological DNA isomers. Type II topoisomerases are ATP-dependent and act by passing a DNA segment through a transient double-strand break. Topoisomerase II is found in phages, archaebacteria, prokaryotes, eukaryotes, and in African Swine Fever virus (ASF). Bacteriophage T4 topoisomerase II consists of three subunits (the product of genes 39, 52 and 60). In prokaryotes and in archaebacteria the enzyme, known as DNA gyrase, consists of two subunits (genes GyrA and GyrB). In some bacteria, a second type II topoisomerase has been identified; it is known as topoisomerase IV and is required for chromosome segregation, it also consists of two subunits (genes parC and parE). In eukaryotes, type II topoisomerase is a homodimer. There are many regions of sequence homology between the different subtypes of topoisomerase II. The signature pattern used in this entry is a highly conserved pentapeptide, which is located in GyrB, in ParE, and in protein 39 of phage T4 topoisomerase.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

9 Found In

Id Name Short Name Type
IPR006171 Toprim domain Toprim_domain Domain
IPR013760 DNA topoisomerase, type IIA-like domain Topo_IIA_like_dom Domain
IPR013759 DNA topoisomerase, type IIA, central domain Topo_IIA_cen_dom Domain
IPR001241 DNA topoisomerase, type IIA Topo_IIA Family
IPR001154 DNA topoisomerase II, eukaryotic-type TopoII_euk Family
IPR005740 DNA topoisomerase 4 subunit B, Gram-positive ParE Family
IPR011557 DNA gyrase, subunit B GyrB Family
IPR005737 DNA topoisomerase IV, subunit B, Gram-negative TopoIV_B_Gneg Family
IPR000565 DNA topoisomerase, type IIA, subunit B Topo_IIA_B Family

0 Parent Features

7 Publications

First Author Title Year Journal Volume Pages
Roca J The mechanisms of DNA topoisomerases. 1995 Trends Biochem Sci 20 156-60
Champoux JJ DNA topoisomerases: structure, function, and mechanism. 2001 Annu Rev Biochem 70 369-413
Gadelle D Phylogenomics of type II DNA topoisomerases. 2003 Bioessays 25 232-42
Wang JC Cellular roles of DNA topoisomerases: a molecular perspective. 2002 Nat Rev Mol Cell Biol 3 430-40
Watt PM Structure and function of type II DNA topoisomerases. 1994 Biochem J 303 ( Pt 3) 681-95
Sternglanz R DNA topoisomerases. 1989 Curr Opin Cell Biol 1 533-5
Sharma A DNA topoisomerases. 1995 Curr Opin Struct Biol 5 39-47

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)