InterPro : IPR002041

Name  Ran GTPase Short Name  Ran_GTPase
Type  Family Description  Small GTPases form an independent superfamily within the larger class ofregulatory GTP hydrolases. This superfamily contains proteins that control avast number of important processes and possess a common, structurallypreserved GTP-binding domain [], []. Sequence comparisons of small G proteinsfrom various species have revealed that they are conserved in primarystructures at the level of 30-55% similarity [].Crystallographic analysis of various small G proteins revealed the presence of a 20 kDa catalytic domain that is unique for the whole superfamily [], []. The domain is built of five alpha helices (A1-A5), sixbeta-strands (B1-B6) and five polypeptide loops (G1-G5). A structuralcomparison of the GTP- and GDP-bound form, allows one to distinguish twofunctional loop regions: switch I and switch II that surround thegamma-phosphate group of the nucleotide. The G1 loop (also called the P-loop)that connects the B1 strand and the A1 helix is responsible for the binding ofthe phosphate groups. The G3 loop provides residues for Mg(2+) and phosphatebinding and is located at the N terminus of the A2 helix. The G1 and G3 loopsare sequentially similar to Walker A and Walker B boxes that are found inother nucleotide binding motifs. The G2 loop connects the A1 helix and the B2strand and contains a conserved Thr residue responsible for Mg(2+) binding.The guanine base is recognised by the G4 and G5 loops. The consensus sequenceNKXD of the G4 loop contains Lys and Asp residues directly interacting withthe nucleotide. Part of the G5 loop located between B6 and A5 acts as arecognition site for the guanine base [].The small GTPase superfamily can be divided in 8 different families:Arf small GTPases. GTP-binding proteins involved in protein trafficking bymodulating vesicle budding and un-coating within the Golgi apparatusRan small GTPases. GTP-binding proteins involved in nucleocytoplasmictransport. Required for the import of proteins into the nucleus and alsofor RNA exportRab small GTPases. GTP-binding proteins involved in vesicular traffic.Rho small GTPases. GTP-binding proteins that control cytoskeletonreorganisationRas small GTPases. GTP-binding proteins involved in signaling pathwaysSar1 small GTPases. Small GTPase component of the coat protein complex II(COPII) which promotes the formation of transport vesicles from theendoplasmic reticulum (ER)Mitochondrial Rho (Miro). Small GTPase domain found in mitochondrialproteins involved in mitochondrial traffickingRoc small GTPases domain. Small GTPase domain always found associated withthe COR domain.Ran (or TC4), is an evolutionary conserved member of the Ras superfamily of small GTPases that regulates all receptor-mediated transport between the nucleus and the cytoplasm. Ran has been implicated in a large number of processes, including nucleocytoplasmic transport, RNA synthesis, processing and export and cell cycle checkpoint control [, ]. Ran plays a crucial role in both import/export pathways and determines the directionality of nuclear transport. Import receptors (importins) bind their cargos in the cytoplasm where the concentration of RanGTP is low (due to action of RanGAP), and release their cargos in the nucleus where the concentration of RanGTP is high (due to action of RanGEF) [, ]. Export receptors (exportins) respond to RanGTP in the opposite manner. Furthermore, it has been shown that nuclear transport factor 2 (NTF2, ) stimulates efficient nuclear import of a cargo protein. NTF2 binds specifically to RanGDP and to the FXFG repeat containing nucleoporins []. Ran is generally included in the RAS 'superfamily' of small GTP-binding proteins [], but it is only slightly related to the other RAS proteins. It also differs from RAS proteins in that it lacks cysteine residues at its C-terminal and is therefore not subject to prenylation. Instead, Ran has an acidic C terminus. It is, however, similar to RAS family members in requiring a specific guanine nucleotide exchange factor (GEF) and a specific GTPase activating protein (GAP) as stimulators of overall GTPase activity.Ran consists of a core domain that is structurally similar to the GTP-binding domains of other small GTPases but, in addition, Ran has a C-terminal extension consisting of an unstructured linker and a 16 residue alpha-helix that is located opposite the "Switch I" region in the RanGDP structure []. Three regions of Ran change conformation depending on the nucleotide bound, the Switch I and II regions, which interact with the bound nucleotide, as well as the C-terminal extension. In RanGDP, the C-terminal extension contacts the core of the protein, while in RanGTP, the extension is extending away from the core, most likely due to a steric clash between the switch I region and the linker part of the C-terminal extension. This suggests that the C-terminal extension in RanGDP is crucial for shielding residues in the core domain and preventing the switch regions from adopting a GTP-like form. This prevents binding of transport factors to RanGDP that would otherwise lead to uncoordinated interaction between importin beta-like proteins and cellular factors.
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11 Publications

First Author Title Year Journal Volume Pages
Steggerda SM Regulation of nuclear import and export by the GTPase Ran. 2002 Int Rev Cytol 217 41-91
Bourne HR The GTPase superfamily: conserved structure and molecular mechanism. 1991 Nature 349 117-27
Lange A Classical nuclear localization signals: definition, function, and interaction with importin alpha. 2007 J Biol Chem 282 5101-5
Valencia A The ras protein family: evolutionary tree and role of conserved amino acids. 1991 Biochemistry 30 4637-48
Paduch M Structure of small G proteins and their regulators. 2001 Acta Biochim Pol 48 829-50
Pai EF Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis. 1990 EMBO J 9 2351-9
Bourne HR The GTPase superfamily: a conserved switch for diverse cell functions. 1990 Nature 348 125-32
Rush MG The small nuclear GTPase Ran: how much does it run? 1996 Bioessays 18 103-12
Scheffzek K Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form. 1995 Nature 374 378-81
Yoneda Y Nucleocytoplasmic protein transport and recycling of Ran. 1999 Cell Struct Funct 24 425-33
Nilsson J The C-terminal extension of the small GTPase Ran is essential for defining the GDP-bound form. 2002 J Mol Biol 318 583-93



To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)