InterPro : IPR014014

Name  RNA helicase, DEAD-box type, Q motif Short Name  RNA_helicase_DEAD_Q_motif
Type  Domain Description  RNA helicases from the DEAD-box family are found in almost all organisms andhave important roles in RNA metabolism such as splicing, RNA transport,ribosome biogenesis, translation and RNA decay. They are enzymes that unwinddouble-stranded RNA molecules in an energy dependent fashion through thehydrolysis of NTP. DEAD-box RNA helicases belong to superfamily 2 (SF2) ofhelicases. As other SF1 and SF2 members they contain seven conserved motifswhich are characteristic of these two superfamilies [].DEAD-box is named after the amino acids of motif II or Walker B (Mg2+-bindingaspartic acid). Besides these seven motifs, DEAD-box RNA helicases contain aconserved cluster of nine amino-acids (the Q motif) with an invariantglutamine located N-terminally of motif I. An additional highly conserved butisolated aromatic residue is also found upstream of these nine residues [].The Q motif is characteristic of and unique to DEAD box family of helicases.It is supposed to control ATP binding and hydrolysis, and therefore itrepresents a potential mechanism for regulating helicase activity.Several structural analyses of DEAD-box RNA helicases have been reported [, ]. The Q motif is located in close proximity to motif I. Theconserved glutamine and aromatic residues interact with the ADP molecule.Some proteins known to contain a Q motif:Eukaryotic initiation factor 4A (eIF4A). An ATP-dependent RNA helicasewhich is a subunit of the eIF4F complex involved in cap recognition andrequired for mRNA binding to ribosome.Various eukaryotic helicases involved in ribosome biogenesis (DBP3, DRS1,SPB4, MAK5, DBP6, DBP7, DBP9, DBP10).Eukaryotic DEAD-box proteins involved in pre-mRNA splicing (Prp5p, Prp28pand Sub2p).DEAD-box proteins required for mitochondrial genome expression (MSS116 andMRH4).Fungi ATP-dependent RNA helicase DHH1. It is required for decapping andturnover of mRNA.Fungi ATP-dependent RNA helicase DBP5. It is involved in nucleo-cytoplasmictransport of poly(A) RNA.Bacterial ATP-dependent RNA helicase rhlB. It is involved in the RNAdegradosome, a multi-enzyme complex important in RNA processing andmessenger RNA degradation.Bacterial cold-shock DEAD box protein A.This entry represents a region stretching from the conserved aromatic residue to one amino acid after the glutamine of the Q motif.
 Feedback

Sequence Features

GO Displayer

Proteins

InterPro protein domain ID --> Contigs

 

Other

0 Child Features

0 Contains

0 Found In

0 Parent Features

4 Publications

First Author Title Year Journal Volume Pages
Story RM Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii. 2001 Proc Natl Acad Sci U S A 98 1465-70
Benz J Crystal structure of the ATPase domain of translation initiation factor 4A from Saccharomyces cerevisiae--the prototype of the DEAD box protein family. 1999 Structure 7 671-9
Koonin EV Autogenous translation regulation by Escherichia coli ATPase SecA may be mediated by an intrinsic RNA helicase activity of this protein. 1992 FEBS Lett 298 6-8
Tanner NK The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis. 2003 Mol Cell 11 127-38



To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)