InterPro : IPR004575

Name  Cdk-activating kinase assemblyfactor MAT1/Tfb3 Short Name  MAT1/Tfb3
Type  Family Description  MAT1/Tfb3 acts as component of the general transcription and DNA repair factor IIH (TFIIH), which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA []. It is a RING finger protein with a characteristic C3HC4 motif located in the N-terminal domain. MAT1/Tfb3 stabilises the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex which then goes on to activate many of the CDK enzymes intimately involved in the cell cycle []. CDK7 forms a stable complex with cyclin H and MAT1/Tfb3 in vivo only when phosphorylated on either one of two residues (Ser164 or Thr170) in its T-loop. The requirement for MAT1/Tfb3 for the activation of CAK can be by-passed by the phosphorylation of CDK7 on the T-loop. The two mechanisms for CDK7 complex stabilisation and activation (MAT1 addition and T-loop phosphorylation), which can operate independently in vitro, actually cooperate under physiological conditions to maintain complex integrity. With prolonged exposure to elevated temperature, dissociation to monomeric subunits occurs in vivo when CDK7 is dephosphorylated, even in the presence of MAT1/Tfb3 [].

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

4 Contains

Id Name Short Name Type
IPR001841 Zinc finger, RING-type Znf_RING Domain
IPR015877 Cdk-activating kinase assembly factor MAT1, centre Cdk-activating_kinase_MAT1_cen Domain
IPR013083 Zinc finger, RING/FYVE/PHD-type Znf_RING/FYVE/PHD Domain
IPR017907 Zinc finger, RING-type, conserved site Znf_RING_CS Conserved_site

0 Found In

0 Parent Features

3 Publications

First Author Title Year Journal Volume Pages
Schultz P Molecular structure of human TFIIH. 2000 Cell 102 599-607
Larochelle S T-loop phosphorylation stabilizes the CDK7-cyclin H-MAT1 complex in vivo and regulates its CTD kinase activity. 2001 EMBO J 20 3749-59
Feaver WJ Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH. Homology to human cyclin-dependent kinase activating kinase and IIH subunits. 1997 J Biol Chem 272 19319-27

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)