InterPro : IPR011010

Name  DNA breaking-rejoining enzyme, catalytic core Short Name  DNA_brk_join_enz
Type  Domain Description  Phage integrases are enzymes that mediate unidirectional site-specific recombination between two DNA recognition sequences, the phage attachment site, attP, and the bacterial attachment site, attB []. Integrases may be grouped into two major families, the tyrosine recombinases and the serine recombinases, based on their mode of catalysis. Tyrosine family integrases, such as Bacteriophage lambdaintegrase, utilise a catalytic tyrosine to mediate strand cleavage, tend to recognise longer attP sequences, and require other proteins encoded by the phage or the host bacteria.The 356 amino acid lambda integrase consists of two domains: an N-terminal domain that includes residues 1-64 and is responsible for binding the arm-type sites of attP, and a C-terminal domain (CTD) that binds the lower affinity core-type sites and contains the catalytic site. The CTD can be further divided into the core-type binding domain (residues 65-169) and the catalytic core domain (170-356), the later representing this entry. The catalytic core adopts an alpha3-beta3-alpha4 fold, where one side of the beta sheet is exposed.The recombinases Cre from phage P1, XerD from Escherichia coliand Flp from yeast are members of the tyrosine recombinase family, and have a two-domain motif resembling that of lambda integrase, as well as sharing a conserved binding mechanism []. The structural fold of their catalytic core domains resemble that of Lambda integrase.The catalytic core of the eukaryotic DNA topoisomerase I shares significant structural similarity with the bacteriophage family of DNA integrases []. Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination.
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Sequence Features

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Proteins

InterPro protein domain ID --> Contigs

 

Other

1 Child Features

Id Name Short Name Type
IPR013762 Integrase-like, catalytic core Integrase-like_cat-core Domain

4 Contains

Id Name Short Name Type
IPR013500 DNA topoisomerase I, catalytic core, eukaryotic-type TopoI_cat_euk Domain
IPR014727 DNA topoisomerase I, catalytic core, alpha/beta subdomain TopoI_cat_a/b-sub_euk Domain
IPR014711 DNA topoisomerase I, catalytic core, alpha-helical subdomain, eukaryotic-type TopoI_cat_a-hlx-sub_euk Domain
IPR018521 DNA topoisomerase I, active site TopoI_AS Active_site

4 Found In

Id Name Short Name Type
IPR001631 DNA topoisomerase I TopoI Family
IPR013499 DNA topoisomerase I, eukaryotic-type TopoI_euk Domain
IPR020876 Tyrosine recombinase XerD-like Tyrosine_recombinase_XerD-like Family
IPR014417 Integrase, predicted Integrase_predicted Family

0 Parent Features

3 Publications

First Author Title Year Journal Volume Pages
Redinbo MR Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA. 1998 Science 279 1504-13
Groth AC Phage integrases: biology and applications. 2004 J Mol Biol 335 667-78
Swalla BM Conservation of structure and function among tyrosine recombinases: homology-based modeling of the lambda integrase core-binding domain. 2003 Nucleic Acids Res 31 805-18



To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)