InterPro : IPR013123

Name  RNA 2-O ribose methyltransferase, substrate binding Short Name  SpoU_subst-bd
Type  Domain Description  Most cellular RNAs undergo a number of post-transcriptional nucleoside modifications. While the biological role of many of these modifications is unknown, some have been shown to be necessary for cell growth or for resistance to antibiotics [, ]. One of the most common modifications is 2'O-ribose methylation catalysed by the RNA 2'O-ribose methyltransferases, a large enzyme family that transfer a methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the backbone ribose [].This entry represents a substrate-binding domain found in a variety of bacterial and mitochondrial RNA 2'-O ribose methyltransferases. These include the bacterial enzyme RlmB, which specifically methylates the conserved nucleotide guanosine 2251 in 23S RNA, and PET56, which specifically methylates the equivalent guanosine in mitochondrial 21S RNA [, ]. This domain forms a four-stranded mixed beta sheet similar to that found in other RNA binding enzymes []. It shows considerable conformational flexibility which is thought to be important for its ability to bind RNA.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

2 Found In

Id Name Short Name Type
IPR004441 RNA methyltransferase TrmH family rRNA_MeTrfase_TrmH Family
IPR016479 tRNA/rRNA methyltransferase, YfiF, predicted YfiF_prd Family

1 Parent Features

Id Name Short Name Type
IPR029064 50S ribosomal protein L30e-like L30e-like Domain

5 Publications

First Author Title Year Journal Volume Pages
Sirum-Connolly K Functional requirement of a site-specific ribose methylation in ribosomal RNA. 1993 Science 262 1886-9
Yu L Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance. 1997 Nat Struct Biol 4 483-9
Michel G The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot. 2002 Structure 10 1303-15
Cavaillé J The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs. 1999 RNA 5 66-81
Lövgren JM The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli. 2001 J Bacteriol 183 6957-60

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)