InterPro : IPR003150

Name  DNA-binding RFX-type winged-helix domain Short Name  DNA-bd_RFX
Type  Domain Description  The RFX family of transcription factors is characterised by a unique approximately 75-residue DNA-binding domain. RFX genes have been isolated in yeasts, nematode and vertebrates. The characteristic RFX-type HTH DNA binding domain has been recruited into otherwise very divergent regulatory factors functioning in a diverse spectrum of unrelated systems, including regulation of the mitotic cell cycle in fission yeast, the control of the immune response in mammals,and infection by human hepatitis B virus [, ]. The RFX-type DNA-binding domain is an unusual member of the winged-helixsubfamily of helix-turn-helix domains because it uses a beta-hairpin (or wing)to recognise DNA instead of the recognition helix typical of helix-tun-helixdomains. It consists of three alpha-helices (H), three beta-strands (S) andthree connecting loops (L), arranged in the order H1-S1-H2-L1-H3-L2-S2-W1-S3.The third loop, connecting beta-strands S2 and S3, forms wing W1 of thewinged-helix motif. In contrast to previously describedwinged-helix DBDs (typically 110 residues long with two wings, W1 and W2), theshorter RFX-type winged-helix DNA-binding domain has only one wing [].

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

0 Found In

1 Parent Features

Id Name Short Name Type
IPR011991 Winged helix-turn-helix DNA-binding domain WHTH_DNA-bd_dom Domain

3 Publications

First Author Title Year Journal Volume Pages
Gajiwala KS Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding. 2000 Nature 403 916-21
Emery P RFX proteins, a novel family of DNA binding proteins conserved in the eukaryotic kingdom. 1996 Nucleic Acids Res 24 803-7
Durand B Cloning and characterization of dRFX, the Drosophila member of the RFX family of transcription factors. 2000 Gene 246 285-93

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)