InterPro : IPR003619

Name  MAD homology 1, Dwarfin-type Short Name  MAD_homology1_Dwarfin-type
Type  Domain Description  Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth []. The dwarfin family also includes the Drosophila protein MAD that is required for the function of decapentaplegic (DPP) and may play a role in DPP signalling. Drosophila Mad binds to DNA and directly mediates activation of vestigial by Dpp []. This domain is also found in nuclear factor I (NF-I) or CCAAT box-binding transcription factor (CTF).This entry represents the MH1 (MAD homology 1) domain is found at the amino terminus of MAD related proteins such as Smads. This domain is separated from the MH2 domain by a non-conserved linker region. The crystal structure of the MH1 domain shows that a highly conserved 11 residue beta hairpin is used to bind the DNA consensus sequence GNCN in the major groove, shown to be vital for the transcriptional activation of target genes. Not all examples of MH1 can bind to DNA however. Smad2 cannot bind DNA and has a large insertion within the hairpin that presumably abolishes DNA binding. A basic helix (H2) in MH1 with the nuclear localisation signal KKLKK has been shown to be essential for Smad3 nuclear import. Smads also use the MH1 domain to interact with transcription factors such as Jun, TFE3, Sp1, and Runx [, , ].
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Sequence Features

GO Displayer

Proteins

InterPro protein domain ID --> Contigs

 

Other

1 Child Features

Id Name Short Name Type
IPR013019 MAD homology, MH1 MAD_homology_MH1 Domain

0 Contains

2 Found In

Id Name Short Name Type
IPR000647 CTF transcription factor/nuclear factor 1 CTF/NFI Family
IPR020604 CTF transcription factor/nuclear factor 1, DNA-binding domain CTF/NFI_DNA-bd-dom Domain

0 Parent Features

3 Publications

First Author Title Year Journal Volume Pages
Kim J Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic. 1997 Nature 388 304-8
Yingling JM Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. 1996 Proc Natl Acad Sci U S A 93 8940-4
Attisano L The Smads. 2001 Genome Biol 2 REVIEWS3010



To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)