InterPro : IPR001506

Name  Peptidase M12A, astacin Short Name  Peptidase_M12A
Type  Domain Description  This group of metallopeptidases belong to the MEROPS peptidase family M12, subfamily M12A (astacin family, clan MA(M)). The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA and the predicted active site residues for members of this family and thermolysin occur in the motif HEXXH [].The astacin () family of metalloendopeptidases encompasses a range of proteinsfound in hydra to humans, in mature and developmental systems []. Theirfunctions include activation of growth factors, degradation of polypeptides,and processing of extracellular proteins []. The proteins are synthesisedwith N-terminal signal and pro-enzyme sequences, and many contain multipledomains C-terminal to the protease domain. They are either secreted fromcells, or are associated with the plasma membrane.The astacin molecule adopts a kidney shape, with a deep active-site cleftbetween its N- and C-terminal domains []. The zinc ion, which lies at thebottom of the cleft, exhibits a unique penta-coordinated mode of binding,involving 3 histidine residues, a tyrosine and a water molecule (which isalso bound to the carboxylate side chain of Glu93) []. The N-terminaldomain comprises 2 alpha-helices and a 5-stranded beta-sheet. The overalltopology of this domain is shared by the archetypal zinc-endopeptidasethermolysin. Astacin protease domains also share common features withserralysins, matrix metalloendopeptidases, and snake venom proteases; theycleave peptide bonds in polypeptides such as insulin B chain and bradykinin,and in proteins such as casein and gelatin; and they have arylamidaseactivity [].

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

7 Found In

Id Name Short Name Type
IPR008294 Meprin alpha/beta subunit Meprin Family
IPR015446 Bone morphogenetic protein 1/tolloid-like protein BMP_1/tolloid-like Family
IPR017369 SPAN protein/blastula protease 10 SPAN/blastula_protease_10 Family
IPR017050 Metallopeptidase, nematode Metallopeptidase_nem Family
IPR017370 Hatching enzyme, Uvs2 Hatching_enzyme_Uvs2 Family
IPR017367 Peptidase M12A, nematode astacin 7/8 Peptidase_M12A_astacin-7/8 Family
IPR017368 Peptidase M12A, nematode astacin 9/10/11 Peptidase_M12A_astacin-9/10/11 Family

1 Parent Features

Id Name Short Name Type
IPR006026 Peptidase, metallopeptidase Peptidase_Metallo Domain

3 Publications

First Author Title Year Journal Volume Pages
Rawlings ND Evolutionary families of metallopeptidases. 1995 Methods Enzymol 248 183-228
Gomis-Rüth FX Refined 1.8 A X-ray crystal structure of astacin, a zinc-endopeptidase from the crayfish Astacus astacus L. Structure determination, refinement, molecular structure and comparison with thermolysin. 1993 J Mol Biol 229 945-68
Bond JS The astacin family of metalloendopeptidases. 1995 Protein Sci 4 1247-61

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)