InterPro : IPR001881

Name  EGF-like calcium-binding domain Short Name  EGF-like_Ca-bd_dom
Type  Domain Description  A sequence of about forty amino-acid residues found in epidermal growth factor (EGF) has been shown [, , , , ]to be present in a large number of membrane-bound and extracellular, mostly animal, proteins. Many of these proteins require calcium for their biological function and a calcium-binding site has been found at the N terminus of some EGF-like domains []. Calcium-binding may be crucial for numerous protein-protein interactions.For human coagulation factor IX it has been shown []that the calcium-ligands form a pentagonal bipyramid. The first, third and fourth conserved negatively charged or polar residues are side chain ligands. The latter is possibly hydroxylated (see aspartic acid and asparagine hydroxylation site) []. A conserved aromatic residue, as well as the second conserved negative residue, are thought to be involved in stabilising the calcium-binding site.As in non-calcium binding EGF-like domains, there are six conserved cysteines and the structure of both types is very similar as calcium-binding induces only strictly local structural changes [].+------------------+ +---------+| | | |nxnnC-x(3,14)-C-x(3,7)-CxxbxxxxaxC-x(1,6)-C-x(8,13)-Cx| | +------------------+'n': negatively charged or polar residue [DEQN]'b': possibly beta-hydroxylated residue [DN]'a': aromatic amino acid'C': cysteine, involved in disulphide bond'x': any amino acid.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

3 Contains

Id Name Short Name Type
IPR013032 EGF-like, conserved site EGF-like_CS Conserved_site
IPR000152 EGF-type aspartate/asparagine hydroxylation site EGF-type_Asp/Asn_hydroxyl_site PTM
IPR018097 EGF-like calcium-binding, conserved site EGF_Ca-bd_CS Conserved_site

5 Found In

Id Name Short Name Type
IPR000742 Epidermal growth factor-like domain EG-like_dom Domain
IPR011398 Fibrillin/Microneme protein4 FBN/EtMIC4 Family
IPR016316 Complement component C1q/Thrombomodulin CD93/CD141 Family
IPR003056 GPCR, family 2, CD97 antigen GPCR_2_CD97 Family
IPR001491 Thrombomodulin Thrombomodulin Family

0 Parent Features

7 Publications

First Author Title Year Journal Volume Pages
Appella E Structure and function of epidermal growth factor-like regions in proteins. 1988 FEBS Lett 231 1-4
Doolittle RF Computer-based characterization of epidermal growth factor precursor. 1984 Nature 307 558-60
Davis CG The many faces of epidermal growth factor repeats. 1990 New Biol 2 410-9
Blomquist MC Vaccinia virus 19-kilodalton protein: relationship to several mammalian proteins, including two growth factors. 1984 Proc Natl Acad Sci U S A 81 7363-7
Rao Z The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions. 1995 Cell 82 131-41
Selander-Sunnerhagen M How an epidermal growth factor (EGF)-like domain binds calcium. High resolution NMR structure of the calcium form of the NH2-terminal EGF-like domain in coagulation factor X. 1992 J Biol Chem 267 19642-9
Barker WC [Similar domains in different proteins: detection and significance]. 1986 Tanpakushitsu Kakusan Koso   54-68

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)