InterPro : IPR006600

Name  HTH CenpB-type DNA-binding domain Short Name  HTH_CenpB_DNA-bd_dom
Type  Domain Description  The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain ofabout 70-75 amino acids, present in eukaryotic centromere proteins andtransposases. The domain is named after the mammalian major centromereautoantigen B or centromere protein B (CENP-B), which is a fundamentalcentromere component of chromosomes. The N terminus of CENP-B contains twoDNA-binding HTH domains, which bind to adjacent major grooves of DNA. TheN terminus of CENP-B is formed by a psq-type HTH domain andC-terminal to this domain lies the CENPB-type HTH domain. These two HTHdomains together bind specifically to a 17-base-pair sequence, the CENP-B box,which occurs in alpha-satellite DNA in human centromeres [].The structure of the CENPB-type HTH domain is composed of three alpha-helices. The second and third helices connected via a turn comprisethe helix-turn-helix motif. Helix 3 is termed the recognition helix as itbinds the DNA major groove, like in other HTHs. In CENP-B this domainrecognises site 3 of the CENP-B box, while the preceding psq-type HTH binds site 1 of the CENP-B box, and a connecting linker loopbinds in the minor groove of DNA and recognises site 2 [].Some proteins known to contain a CENPB-type HTH domain:Mammalian centromere protein B (CENP-B), associated with the centromere and specifically binding DNA to the CENP-B box.Mammalian jerky protein, involved in epileptic seizures in mice [].Mammalian Pogo transposases []and tigger transposable elements [].Fission yeast ARS-binding protein 1 (abp1) []and CENP-B homologue proteins (CBHP-1 and 2), which are centromere proteins [, ].Candida albicans protein PDC2 (Pyruvate DeCarboxylase 2) [, ].Fungal transposases.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

0 Found In

1 Parent Features

Id Name Short Name Type
IPR009057 Homeodomain-like Homeodomain-like Domain

10 Publications

First Author Title Year Journal Volume Pages
Tanaka Y Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA. 2001 EMBO J 20 6612-8
Tanaka Y Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences. 2005 J Biol Chem 280 41609-18
Kipling D Centromeres, CENP-B and Tigger too. 1997 Trends Genet 13 141-5
Wang H Pogo transposase contains a putative helix-turn-helix DNA binding domain that recognises a 12 bp sequence within the terminal inverted repeats. 1999 Nucleic Acids Res 27 455-61
Dou T Isolation and characterization of a Jerky and JRK/JH8 like gene, tigger transposable element derived 7, TIGD7. 2004 Biochem Genet 42 279-85
Hohmann S Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevisiae. 1993 Mol Gen Genet 241 657-66
Nosaka K Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae. 2008 FEBS Lett 582 3991-6
Locovei AM The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast. 2006 Cell Div 1 27
Lee JK Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe. 1997 Proc Natl Acad Sci U S A 94 8427-32
Irelan JT Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. 2001 Genetics 157 1191-203

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)