InterPro : IPR013422

Name  CRISPR-associated protein Cas5, N-terminal Short Name  CRISPR-assoc_prot_Cas5_N
Type  Domain Description  The CRISPR-Cas system is a prokaryotic defense mechanism against foreign genetic elements. The key elements of this defense system are the Cas proteins and the CRISPR RNA. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a family of DNA direct repeats separated by regularly sized non-repetitive spacer sequences that are found in most bacterial and archaeal genomes []. CRISPRs appear to provide acquired resistance against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).The defense reaction is divided into three stages. In the adaptation stage, the invader DNA is cleaved, and a piece of it is selected to be integrated as a new spacer into the CRISPR locus, where it is stored as an identity tag for future attacks by this invader. During the second stage (the expression stage), the CRISPR RNA (pre-crRNA) is transcribed and subsequently processed into the mature crRNAs. In the third stage (the interference stage), Cas proteins, together with crRNAs, identify and degrade the invader [, , ].The CRISPR-Cas systems have been sorted into three major classes. In CRISPR-Cas types I and III, the mature crRNA is generally generated by a member of the Cas6 protein family. Whereas in system III the Cas6 protein acts alone, in some class I systems it is part of a complex of Cas proteins known as Cascade (CRISPR-associated complex for antiviral defense). The Cas6 protein is necessary for crRNA production whereas the additional Cas proteins that form the Cascade complex are needed for crRNA stability []. This entry represents a shared N-terminal region, of about 43 amino acids in length, found in a number of Cas proteins. This region is widely distributed and was designated Cas5. Proteins containing this region are generally 210 to 265 amino acids in length and show little or no homology between their C-terminal regions. The best characterised protein in this entry is DevS () from Myxococcus xanthus, a Cas protein that appears to participate in a species-specific developmental pathway. Cas5 is found within a cluster of three cas genes associated with CRISPR structures in many bacterial species, named cas1B, cas5 and cas6 [].

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

0 Contains

7 Found In

Id Name Short Name Type
IPR010147 CRISPR-associated protein, CasD CRISPR-assoc_prot_CasD Family
IPR021124 CRISPR-associated protein, Cas5 CRISPR-assoc_prot_Cas5 Family
IPR010153 CRISPR-associated protein, Cas5a type CRISPR-assoc_prot_Cas5a-typ Family
IPR010155 CRISPR-associated protein, Cas5d-type CRISPR-assoc_prot_Cas5d-typ Family
IPR013415 CRISPR-associated protein Cas5, bacterial Cas5_bac Family
IPR013421 CRISPR-associated protein Cas5, HALMA CRISPR-assoc_prot_Cas5_HALMA Family
IPR013337 CRISPR-associated protein Cas5, Tneap type CRISPR-assoc_prot_Cas5_Tneap Family

0 Parent Features

6 Publications

First Author Title Year Journal Volume Pages
Kunin V Evolutionary conservation of sequence and secondary structures in CRISPR repeats. 2007 Genome Biol 8 R61
Barrangou R CRISPR provides acquired resistance against viruses in prokaryotes. 2007 Science 315 1709-12
Makarova KS A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. 2006 Biol Direct 1 7
Howard JA Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein. 2011 Biochem J 439 85-95
Brendel J A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii. 2014 J Biol Chem 289 7164-77
Bolotin A Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. 2005 Microbiology 151 2551-61

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)