InterPro : IPR005917

Name  Phosphomevalonate kinase, Gram-positive Short Name  Pmev_kin_Gpos
Type  Family Description  This entry represents a group of predicted phosphomevalonate kinases from Gram-positive bacteria. Phosphomevalonate kinase ()catalyzes the phosphorylation of 5-phosphomevalonate into 5-diphosphomevalonate, an essential step in isoprenoid biosynthesis via the mevalonate pathway. This pathway starts with three molecules of acetyl-CoA, which, in a series of six different enzyme reactions, are converted to isopentanyl pyrophosphate (IPP), the basic C5 isoprene unit and a common intermediate for a number of pathways including isoprenoid and cholesterol biosynthesis.There are two unrelated types of PMK. The first type belongs to the GHMP kinase and includes a eukaryotic group which is typified by Saccharomyces cerevisiaeERG8 []and a bacterial group typified by by Streptococcus pneumoniaePMK (MvaK2) []. The second type includes animal PMKs, typified by human PMK []. PMKs of the first type are present in eubacteria, fungi, and plants, while the second type is found only in animals, indicative of a nonorthologous gene displacement early in animal evolution []. PMKs of the GHMP kinase type are closely related to mevalonate kinases. The two types of PMK have different consensus ATP-binding motifs: a protein kinase motif in the ERG8 orthologs versus a P-loop or Walker A motif in the animal orthologs. The fact that ERG8 orthologs are found in pathogenic eubacteria and fungi but not in humans makes them attractive targets for the development of antibacterial and/or antifungal drugs [].This group of enzymes belongs to the GHMP kinase domain superfamily. GHMP kinases are a unique class of ATP-dependent enzymes (the abbreviation of which refers to the original members: galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase) []. Enzymes belonging to this superfamily contain three well-conserved motifs, the second of which has the typical sequence Pro-X-X-X-Gly-Leu-X-Ser-Ser-Ala and is involved in ATP binding []. The phosphate binding loop in GHMP kinases is distinct from the classical P-loops found in many ATP/GTP binding proteins. The bound ADP molecule adopts a rare syn conformation and is in the opposite orientation from those bound to the P-loop-containing proteins []. GHMP kinases display a distinctly bilobal appearance with their N-terminal subdomains dominated by a mixed beta-sheet flanked on one side by alpha-helices and their C-terminal subdomains containing a four stranded anti-parallel beta-sheet [, , , ].

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

4 Contains

Id Name Short Name Type
IPR020568 Ribosomal protein S5 domain 2-type fold Ribosomal_S5_D2-typ_fold Domain
IPR013750 GHMP kinase, C-terminal domain GHMP_kinase_C_dom Domain
IPR006204 GHMP kinase N-terminal domain GHMP_kinase_N_dom Domain
IPR014721 Ribosomal protein S5 domain 2-type fold, subgroup Ribosomal_S5_D2-typ_fold_subgr Domain

0 Found In

0 Parent Features

8 Publications

First Author Title Year Journal Volume Pages
Tsay YH Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase. 1991 Mol Cell Biol 11 620-31
Houten SM Nonorthologous gene displacement of phosphomevalonate kinase. 2001 Mol Genet Metab 72 273-6
Zhou T Structure and mechanism of homoserine kinase: prototype for the GHMP kinase superfamily. 2000 Structure 8 1247-57
Wada T Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis. 2003 J Biol Chem 278 30022-7
Bork P Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases. 1993 Protein Sci 2 31-40
Romanowski MJ Crystal structure of the Streptococcus pneumoniae phosphomevalonate kinase, a member of the GHMP kinase superfamily. 2002 Proteins 47 568-71
Chambliss KL Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence. 1996 J Biol Chem 271 17330-4
Thoden JB Molecular structure of galactokinase. 2003 J Biol Chem 278 33305-11

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)