InterPro : IPR003991

Name  Pertactin virulence factor family Short Name  Pertactin_virulence_factor
Type  Family Description  Bordetella pertussisis a Gram-negative, aerobic coccobacillus that causes pertussis (whooping cough), especially in young children []. Once present in the lungs, the bacterium attaches to ciliated pulmonary epithelial cells via a collection of outer membrane proteins, all of which are virulence factors.Pertactin, or P69 protein, is one of these virulence factors. Pertactin andfilamentous haemagglutinin have been identified as Bordetella adhesins []. Both proteins contain an arg-gly-asp (RGD) motif that promotes binding to integrins, known to be important in cell mobility and development. Theproduction of most Bordetella virulence factors (including pertactin) is controlled by a two-component signal transduction system, comprising theBvgA regulator and the BvgS sensor []. Pertactin shares a high level of similarity with other Bordetella adhesins, such as BrkA. The protein isfirst produced as a 93kDa precursor. Upon secretion into the extracellularenvironment, a 30kDa domain at the C terminus remains in the outer membrane,while the mature 60.4kDa pertactin molecule is released [].The crystal structure of mature pertactin has been determined to 2.5A resolution by means of X-ray diffraction. The fold is characterised by a 16-stranded parallel beta-helix, with a V-shaped cross-section. Several between-strand amino-acid repeats form internal and external ladders. The helical structure is interrupted by several protruding loops that contain motifs associated with the activity of the protein. One such sequence - [GGXXP]5 - appears directly after the RGD motif, and may mediate interaction with epithelial cells. The C-terminal region of P.69 pertactin contains a [PQP]5 motif loop, which contains the major immunoprotective epitope [].The tcfA gene of B. pertussis encodes a unique virulence-associated factor, Tcf (tracheal colonisation factor) []. The derived amino acid sequence of tcfA predicts a 68kDa RGD-containing, proline-rich protein.Amino acid sequence analysis reveals that the C-terminal 30kDa of this protein shares ~50% identity with the 30kDa C terminus of the Bordetella pertactin precursor.The Bordetella resistance to killing, brk, locus has cloned and sequencedand found to encode two divergently transcribed open reading frames (ORFs),designated BrkA and BrkB []. Both ORFs are necessary for serum resistance. BrkA shares ~29% identity with pertactin and contains two RGD motifs, in addition to a proteolytic processing site and an outer membrane targeting signal. BrkA, like pertactin, is involved in adherence and invasion. This entry is the C-terminal end of pertactin and is found associated with the N-terminal section .
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Sequence Features

GO Displayer

Proteins

InterPro protein domain ID --> Contigs

 

Other

1 Child Features

Id Name Short Name Type
IPR003992 Pertactin Pertactin Family

5 Contains

Id Name Short Name Type
IPR011050 Pectin lyase fold/virulence factor Pectin_lyase_fold/virulence Domain
IPR005546 Autotransporter beta-domain Auto_transptbeta Domain
IPR004899 Pertactin, central region Pertactin_central Domain
IPR006315 Outer membrane autotransporter barrel OM_autotransptr_brl Domain
IPR012332 P22 tailspike C-terminal domain-like P22_tailspike_C-like Domain

0 Found In

0 Parent Features

6 Publications

First Author Title Year Journal Volume Pages
Charles IG Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis. 1989 Proc Natl Acad Sci U S A 86 3554-8
Li J Cloning, nucleotide sequence and heterologous expression of the protective outer-membrane protein P.68 pertactin from Bordetella bronchiseptica. 1992 J Gen Microbiol 138 Pt 8 1697-705
Locht C Molecular aspects of Bordetella pertussis pathogenesis. 1999 Int Microbiol 2 137-44
Emsley P Structure of Bordetella pertussis virulence factor P.69 pertactin. 1996 Nature 381 90-2
Finn TM Tracheal colonization factor: a Bordetella pertussis secreted virulence determinant. 1995 Mol Microbiol 16 625-34
Fernandez RC Cloning and sequencing of a Bordetella pertussis serum resistance locus. 1994 Infect Immun 62 4727-38



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Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)