InterPro : IPR014209

Name  RNA polymerase sigma-K type Short Name  RNA_pol_sigma-K
Type  Family Description  The bacterial core RNA polymerase complex, which consists of five subunits, is sufficient for transcription elongation and termination but is unable to initiate transcription. Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme []. RNA polymerase recruits alternative sigma factors as a means of switching on specific regulons. Most bacteria express a multiplicity of sigma factors. Two of these factors, sigma-70 (gene rpoD), generally known as the major or primary sigma factor, and sigma-54 (gene rpoN or ntrA) direct the transcription of a wide variety of genes. The other sigma factors, known as alternative sigma factors, are required for the transcription of specific subsets of genes.With regard to sequence similarity, sigma factors can be grouped into two classes, the sigma-54 and sigma-70 families. Sequence alignments of the sigma70 family members reveal four conserved regions that can be further divided into subregions eg. sub-region 2.2, which may be involved in the binding of the sigma factor to the core RNA polymerase; and sub-region 4.2, which seems to harbor a DNA-binding 'helix-turn-helix' motif involved in binding the conserved -35 region of promoters recognised by the major sigma factors [, ]. The sporulation-specific transcription factor sigma-K (also called sigma-27) is expressed in the mother cell compartment of endospore-forming bacteria such as Bacillus subtilis. Like its close homologue sigma-E (sigma-29, see ), also specific to the mother cell compartment, it must be activated by a proteolytic cleavage.Note: that in B. subtilis (and apparently also in Clostridium tetani), but not in other endospore forming species such as Bacillus anthracis, the sigK gene is generated by a non-germline (mother cell only) chromosomal rearrangement that recombines coding regions for the N-terminal and C-terminal regions of sigma-K [].
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Sequence Features

GO Displayer

Proteins

InterPro protein domain ID --> Contigs

 

Other

0 Child Features

6 Contains

Id Name Short Name Type
IPR011991 Winged helix-turn-helix DNA-binding domain WHTH_DNA-bd_dom Domain
IPR013324 RNA polymerase sigma factor, region 3/4 RNA_pol_sigma_r3_r4 Domain
IPR013325 RNA polymerase sigma factor, region 2 RNA_pol_sigma_r2 Domain
IPR007627 RNA polymerase sigma-70 region 2 RNA_pol_sigma70_r2 Domain
IPR000943 RNA polymerase sigma-70 RNA_pol_sigma70 Domain
IPR007630 RNA polymerase sigma-70 region 4 RNA_pol_sigma70_r4 Domain

0 Found In

1 Parent Features

Id Name Short Name Type
IPR016263 RNA polymerase sporulation-specific sigma factor, SigK/SigE RNA_pol_sigma-SigE/K Family

4 Publications

First Author Title Year Journal Volume Pages
Helmann JD Structure and function of bacterial sigma factors. 1988 Annu Rev Biochem 57 839-72
Lonetto M The sigma 70 family: sequence conservation and evolutionary relationships. 1992 J Bacteriol 174 3843-9
Gribskov M Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. 1986 Nucleic Acids Res 14 6745-63
Kunkel B The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene. 1990 Genes Dev 4 525-35



To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)