InterPro : IPR006474

Name  Helicase Cas3, CRISPR-associated, core Short Name  Helicase_Cas3_CRISPR-ass_core
Type  Domain Description  The CRISPR-Cas system is a prokaryotic defense mechanism against foreign genetic elements. The key elements of this defense system are the Cas proteins and the CRISPR RNA. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a family of DNA direct repeats separated by regularly sized non-repetitive spacer sequences that are found in most bacterial and archaeal genomes []. CRISPRs appear to provide acquired resistance against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).The defense reaction is divided into three stages. In the adaptation stage, the invader DNA is cleaved, and a piece of it is selected to be integrated as a new spacer into the CRISPR locus, where it is stored as an identity tag for future attacks by this invader. During the second stage (the expression stage), the CRISPR RNA (pre-crRNA) is transcribed and subsequently processed into the mature crRNAs. In the third stage (the interference stage), Cas proteins, together with crRNAs, identify and degrade the invader [, , ].The CRISPR-Cas systems have been sorted into three major classes. In CRISPR-Cas types I and III, the mature crRNA is generally generated by a member of the Cas6 protein family. Whereas in system III the Cas6 protein acts alone, in some class I systems it is part of a complex of Cas proteins known as Cascade (CRISPR-associated complex for antiviral defense). The Cas6 protein is necessary for crRNA production whereas the additional Cas proteins that form the Cascade complex are needed for crRNA stability []. This entry represents a highly conserved core region found in the Cas3 family of proteins. These proteins are found in association with CRISPR repeat elements in a broad range of bacteria and archaea []. Cas3 is one of four protein families (Cas1 to Cas4) that are associated with CRISPR elements and always occur near a repeat cluster, usually in the order cas3-cas4-cas1-cas2. Cas3 proteins have motifs characteristic of helicases from superfamily 2 and contain a DEAD/DEAH box region and a conserved C-terminal domain. Some but not all Cas3 family members have an N-terminal HD domain region (), although these sequences are not included within this group. These Cas proteins may be involved in DNA metabolism or gene expression [].
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Sequence Features

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Proteins

InterPro protein domain ID --> Contigs

 

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0 Child Features

2 Contains

Id Name Short Name Type
IPR001650 Helicase, C-terminal Helicase_C Domain
IPR014001 Helicase superfamily 1/2, ATP-binding domain Helicase_ATP-bd Domain

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0 Parent Features

6 Publications

First Author Title Year Journal Volume Pages
Kunin V Evolutionary conservation of sequence and secondary structures in CRISPR repeats. 2007 Genome Biol 8 R61
Barrangou R CRISPR provides acquired resistance against viruses in prokaryotes. 2007 Science 315 1709-12
Makarova KS A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. 2006 Biol Direct 1 7
Howard JA Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein. 2011 Biochem J 439 85-95
Brendel J A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii. 2014 J Biol Chem 289 7164-77
Jansen R Identification of genes that are associated with DNA repeats in prokaryotes. 2002 Mol Microbiol 43 1565-75



To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)