InterPro : IPR005737

Name  DNA topoisomerase IV, subunit B, Gram-negative Short Name  TopoIV_B_Gneg
Type  Family Description  DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single- or double-strand breaks, crossing the strands through one another, then resealing the breaks []. These enzymes have several functions: to remove DNA supercoils during transcription and DNA replication; for strand breakage during recombination; for chromosome condensation; and to disentangle intertwined DNA during mitosis [, ]. DNA topoisomerases are divided into two classes: type I enzymes (; topoisomerases I, III and V) break single-strand DNA, and type II enzymes (; topoisomerases II, IV and VI) break double-strand DNA [].Type II topoisomerases are ATP-dependent enzymes, and can be subdivided according to their structure and reaction mechanisms: type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as for introducing both negative and positive supercoils [].This entry represents subunit B (parE) of topoisomerase IV from Gram negative bacteria. Topoisomerase IV primarily decatenates DNA and relaxes positive supercoils, which is important in bacteria, where the circular chromosome becomes catenated, or linked, during replication []. Topoisomerase IV consists of two polypeptide subunits, parC (subunit A), which is homologous to gyrA of topoisomerase II, and parE (subunit B), which is homologous to gyrB of topoisomerase II.

Sequence Features

GO Displayer


InterPro protein domain ID --> Contigs



0 Child Features

7 Contains

Id Name Short Name Type
IPR020568 Ribosomal protein S5 domain 2-type fold Ribosomal_S5_D2-typ_fold Domain
IPR003594 Histidine kinase-like ATPase, C-terminal domain HATPase_C Domain
IPR013506 DNA topoisomerase, type IIA, subunit B, domain 2 Topo_IIA_bsu_dom2 Domain
IPR013760 DNA topoisomerase, type IIA-like domain Topo_IIA_like_dom Domain
IPR013759 DNA topoisomerase, type IIA, central domain Topo_IIA_cen_dom Domain
IPR018522 DNA topoisomerase, type IIA, conserved site TopoIIA_CS Conserved_site
IPR002288 DNA gyrase B subunit, C-terminal DNA_gyrase_B_C Domain

0 Found In

1 Parent Features

Id Name Short Name Type
IPR001241 DNA topoisomerase, type IIA Topo_IIA Family

6 Publications

First Author Title Year Journal Volume Pages
Roca J The mechanisms of DNA topoisomerases. 1995 Trends Biochem Sci 20 156-60
Champoux JJ DNA topoisomerases: structure, function, and mechanism. 2001 Annu Rev Biochem 70 369-413
Gadelle D Phylogenomics of type II DNA topoisomerases. 2003 Bioessays 25 232-42
Wang JC Cellular roles of DNA topoisomerases: a molecular perspective. 2002 Nat Rev Mol Cell Biol 3 430-40
Watt PM Structure and function of type II DNA topoisomerases. 1994 Biochem J 303 ( Pt 3) 681-95
Corbett KD The structural basis for substrate specificity in DNA topoisomerase IV. 2005 J Mol Biol 351 545-61

To cite PlanMine, please refer to the following publication:

Rozanski, A., Moon, H., Brandl, H., Martín-Durán, J. M., Grohme, M., Hüttner, K., Bartscherer, K., Henry, I., & Rink, J. C.
PlanMine 3.0—improvements to a mineable resource of flatworm biology and biodiversity
Nucleic Acids Research, gky1070. doi:10.1093/nar/gky1070 (2018)